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UPSC Civil ServicesStage 2: Main Examination

Botany

About this optional

Botany is one of the optional subjects of the Civil Services (Main) Examination. A candidate who chooses it writes two papers on it, and both are counted for merit.

PaperMarksDuration
Paper VI: Botany Paper I2503 hours
Paper VII: Botany Paper II2503 hours
  • Both papers are of conventional (essay) type. Together they carry 500 of the 1750 marks of the written examination.
  • The scope of the syllabus is broadly of honours degree level: higher than a bachelor's degree and lower than a master's degree.
  • The question papers are set in Hindi and English. The candidate may answer in English or in any one language of the Eighth Schedule to the Constitution.
  • The optional papers are taken into account only if the candidate gets 25% in each of the qualifying papers, Indian Language and English.

The rules common to every optional are on the Optional Subject (two papers) page. Below is the complete official syllabus of both papers, as the notice gives it.

Paper I

1. Microbiology and Plant Pathology

Structure and reproduction/multiplication of viruses, viroids, bacteria, fungi and mycoplasma; Applications of microbiology in agriculture, industry, medicine and in control of soil and water pollution; Prion and Prion hypothesis.

Important crop diseases caused by viruses, bacteria, mycoplasma, fungi and nematodes; Modes of infection and dissemination; Molecular basis of infection and disease resistance/defence; Physiology of parasitism and control measures. Fungal toxins. Modelling and disease forecasting; Plant quarantine.

2. Cryptogams

Algae, fungi, lichens, bryophytes, pteridophytes-structure and reproduction from evolutionary viewpoint; Distribution of Cryptogams in India and their ecological and economic importance.

3. Phanerogams

Gymnosperms : Concept of Progymnosperms. Classification and distribution of gymnosperms. Salient features of Cycadales, Ginkgoales, Coniferales and Gnetales, their structure and reproduction. General account of Cycadofilicales, Bennettitales and Cordiaitailes; Geological time scale; Type of fossils and their study techniques.

Angiosperms : Systematics, anatomy, embryology, palynology and phylogency.

Taxonomic hierarchy; International Code of Botanical Nomenclature; Numerical taxonomy and chemotaxonomy; Evidence from anatomy, embryology and palynology.

Origin and evolution of angiosperms; Comparative account of various systems of classification of angiosperms; Study of angiospermic families— Magnoliaceae, Ranunculaceae, Brassicaceae, Rosaceae, Fabaceae, Euphorbiaceae, Malvaceae, Dipterocarpaceae, Apiaceae, Asclepiadaceae, Verbenaceae, Solanaceae, Rubiaceae, Cucurbitaceae, Asteraceae, Poaceae, Arecaceae, Liliaceae, Musaceae and Orchidaceae.

Stomata and their types; Glandular and non-glandular trichomes; Unusual secondary growth; Anatomy of C3 and C4 plants; Xylem and phloem differentiation; Wood anatomy.

Development of male and female gametophytes, pollination, fertilization; Endosperm—its development and function. Patterns of embryo development; Polyembroyony, apomixes; Applications of palynology; Experimental embryology including pollen storage and test-tube fertilization.

4. Plant Resource Development

Domestication and introduction of plants; Origin of cultivated plants, Vavilov’s centres of origin. Plants as sources for food, fodder, fibres, spices, beverages, edible oils, drugs, narcotics, insecticides, timber, gums, resins and dyes; latex, cellulose, starch and its products; Perfumery; Importance of Ethnobotany in Indian context; Energy plantations; Botanical Gardens and Herbaria.

5. Morphogenesis

Totipotency, polarity, symmetry and differentiation; Cell, tissue, organ and protoplast culture. Somatic hybrids and Cybrids; Micropropagation; Somaclonal variation and its applications; Pollen haploids, embryo rescue methods and their applications.

Paper II

1. Cell Biology

Techniques of cell biology. Prokaryotic and eukaryotic cells—structural and ultrastructural details; Structure and function of extracellular matrix (cell wall) and membranes-cell adhesion, membrane transport and vesicular transport; Structure and function of cell organelles (chloroplasts, mitochondria, ER, dictyosomes ribosomes, endosomes, lysosomes, peroxisomes; Cytoskelaton and microtubules; Nucleus, nucleolus, nuclear pore complex; Chromatin and nucleosome; Cell signalling and cell receptors; Signal transduction Mitosis and meiosis; molecular basis of cell cycle. Numerical and structural variations in chromosomes and their significance; Chromatin organization and packaging of genome; Polytene chromosomes; B-chromosomes—structure, behaviour and significance.

2. Genetics, Molecular Biology and Evolution

Development of genetics, and gene versus allele concepts (Pseudoalleles); Quantitative genetics and multiple factors; Incomplete dominance, polygenic inheritance, multiple alleles; Linkage and crossing over of gene mapping including molecular maps (idea of mapping, function); Sex chromosomes and sex-linked inheritance; sex determination and molecular basis of sex differentiation; Mutations (biochemical and molecular basis); Cytoplasmic inheritance and cytoplasmic genes (including genetics of male sterility).

Structure and synthesis of nucleic acids and proteins; Genetic code and regulation of gene expression; Gene silencing; Multigene families; Organic evolution-evidences, mechanism and theories. Role of RNA in origin and evolution.

3. Plant Breeding, Biotechnology and Biostatistics

Methods of plant breeding—introduction, selection and hybridization (pedigree, backcross, mass selection, bulk method); Mutation, polyploidy, male sterility and heterosis breeding. Use of apomixes in plant breeding; DNA sequencing; Genetic engineering—methods of transfer of genes; Transgenic crops and biosafety aspects; Development and use of molecular markers in plant breeding; Tools and techniques—probe, southern blotting, DNA fingerprinting, PCR and FISH. Standard deviation and coefficient of variation (CV). Tests of significance (Z-test, t-test and chi-square tests). Probability and distributions (normal, binomial and Poisson). Correlation and regression.

4. Physiology and Biochemistry

Water relations, mineral nutrition and ion transport, mineral deficiencies. Photosynthesis—photochemical reactions, photophosphorylation and carbon fixation pathways; C3, C4 and CAM pathways; Mechanism of phloem transport, Respiration (anaerobic and aerobic, including fermentation)—electron transport chain and oxidative phosphorylation; Photorespiration; Chemiosmotic theory and ATP synthesis; Lipid metabolism; Nitrogen fixation and nitrogen metabolism. Enzymes, coenzymes; Energy transfer and energy conservation. Importance of secondary metabolites. Pigments as photoreceptors (plastidial pigments and phytochrome). Plant movements; Photoperiodism and flowering, vernalization, senescence; Growth substances—their chemical nature, role and applications in agri-horticulture; growth indices, growth movements. Stress physiology (heat, water, salinity, metal); Fruit and seed physiology. Dormancy, storage and germination of seed. Fruit ripening—its molecular basis and manipulation.

5. Ecology and Plant Geography

Concept of ecosystem; Ecological factors. Concepts and dynamics of community; Plant succession. Concepts of biosphere; Ecosystems; Conservation; Pollution and its control (including phytoremediation); Plant indicators; Environment (Protection) Act.

Forest types of India—‘Ecological and economic importance of forests, afforestation, deforestation and social forestry; Endangered plants, endemism IUCN categories, Red Data Books; Biodiversity and its conservation; Protected Area Network; Convention of Biological Diversity, Farmers’ Rights; and Intellectual Property Rights; Concept of Sustainable Development; Biogeochemical cycles. Global warming and climatic change; Invasive species; Environmental Impact Assessment; Phytogeographical regions of India.

Overlaps with General Studies

  • General Studies III shares much of Paper II's ecology part: conservation, biodiversity, environmental pollution and degradation, and environmental impact assessment. Its science and technology part names biotechnology and intellectual property rights, which meet Paper II's genetic engineering, transgenic crops, Farmers' Rights and Intellectual Property Rights lines.
  • Prelims General Studies Paper I asks about general science, environmental ecology, biodiversity and climate change, where the subject's basics help directly.
  • General Studies I touches the forest types and phytogeographical regions of India through its geography of natural resources and of changes in flora and fauna.

Source: UPSC Examination Notice No. 05/2026-CSE, Civil Services Examination, 2026, 4 February 2026, Appendix I, Section III, Part B, PDF. Last checked 11 November 2026.

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